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The antibody is from purified rabbit serum by affinity purification by means of sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene continues to be noticed in several tumors, which results in chromosome instability, and so might contribute to tumorigenesis. This protein was found to affiliate with, and become involved with, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in cell-cycle regulated histone gene expression and performs a critical job in marketing cell-cycle progression inside the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, have been explained. Two extra splice variants ended up described but thorough nucleotide sequence info is not really nevertheless available.
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Blocking peptides are peptides that bind especially on the focus on antibody and block antibody binding. These peptide normally has the epitope regarded by the antibody.
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This tutorial outlines even further attainable optimization of cellular and immunoassay parameters to ensure the very best benefits are...
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This information outlines even further doable optimization of mobile and immunoassay parameters to guarantee the very best effects are...
Antibodies sure to the blocking peptide now not bind to your epitope to the focus on protein. By evaluating the staining through the blocked antibody compared to the antibody on your own, one can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs to your remarkably conserved cyclin household, whose associates are characterized by a extraordinary periodicity in protein abundance in the mobile cycle. Cyclins functionality as regulators of CDK kinases. Distinct cyclins show unique expression and degradation designs which add on the temporal coordination of each mitotic party. Cyclin E varieties a complex with and capabilities for a regulatory subunit of CDK2, whose action is necessary for mobile cycle G1/S transition. This protein accumulates within the G1-S stage boundary and is degraded as cells development by means of S section.
The measurement of protein phosphorylation get more info is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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